Umeå Metabolomics
Facility/Node
Umeå University Metabolomics Facility
Contact person
Thomas Moritz Thomas.moritz@genfys.slu.se
Facility website:
http://www.upsc.se/index.php?option=com_content&task=blogcategory&id=66&Itemid=90
Summary of service
Techniques enabling metabolites to be identified and metabolic fluxes to be quantified are essential to complement the information provided by genetic experiments and the large-scale analysis of transcript and protein profiles in living organisms. The main services at the facility are the following:
- Advice regarding design of metabolomics experiment
- Advice regarding extraction protocols
- Extraction of samples (depends on the type of samples)
- Mass spectrometry analysis
- Basic multivariate analysis on obtained results (PCA, PLS)
- Simple presentation of obtained results. Only the differences between samples will be presented
The services at the facility will not include:
- Extensive identification of unknown compounds.
- Extensive multivariate analysis on obtained results.
Technologies/Methods
Instrumentation at the metabolomics facility:
Mass spectrometers funded by WCN, and dedicated to metabolomics
LECO Pegasus III, Gas chromatography - mass spectrometry/time-of-flight analyser (GC/TOF)
Bruker Esquire 3000 plus, Liquid chromatography – mass spectrometer (LC/MS)
LC/TOFMS (available summer 2005)
Mass spectrometers at the UPSC mass spectrometer facility:
JEOL JMS-MStation GC/MS (magnetic-sector mass spectrometer)
JEOL JMS-SX/SX102 GC/MS (four-sector tandem mass spectrometer)
Micromass Quattro Ultima LC/MS (triple-stage quadrupole mass spectrometer)
Micromass Q-TOF Ultima LC/MS (quadrupole/time-of-flight mass spectrometer)
Applied Biosystem Voyager-DE STR (Maldi-TOF)
Above instruments might, when necessary, be used within the metabolomics facility.
Practical information
For more practical information about the services we offer and prices see our website.
Details/theories on technology
