Uppsala Array

Facility/Node
Uppsala Array Platform

Contact person
Anders Isaksson [anders.isaksson@genpat.uu.se]

Facility website: http://www.medsci.uu.se/klinfarm/arrayplatform/ 

Summary of service
Uppsala Array Platform was setup as collaboration between Uppsala University and the Akademiska laboratory. We function as an array core facility providing access to microarray technology for the research community. We offer all types of services from the initial consultation, performing the complete array experiments to analyzing the results. Contact us for more information!
The research applications we provide are for example gene expression analysis, investigation of copy number variation on a genomic level, and ChIP-chip experiments. We also provide diagnostic analysis using array-based methods. Practical courses on data analysis, demonstrations and other types of support are also available.

Technologies/Methods
We are focusing on using the commercially available Affymetrix technology, but we also have several years experience using both one- and two-channel cDNA and oligonucleotide-based arrays for expression analysis. 
-The Affymetrix technology
GeneChip probe arrays are manufactured using technology that
combines photolithography and combinatorial chemistry. Up to 1.3
million different oligonucleotide probes are synthesized on each
array. Each probe cell contains hundreds of thousands to millions of copies of a given oligonucleotide.
- Expression analysis using Codelink bioarrays
We have experience from several types of spotted arrays, both cDNA and oligonucleotide based, but in order to provide a flexible and high quality platform for our customers the technique we offer today is Codelink Bioarrays manufactured by GE Healthcare.
 We offer all types of services from the initial consultation,
performing the complete array experiments to analyzing the results.
Since we are focusing on using the commercially available Affymetrix technology we provide all types of analysis for the applications available. We also have several years experience using both one- and two-channel cDNA and oligonucleotide-based arrays for expression analysis, so we can offer service and support on all steps of the analysis procedure here including experimental design, pre-processing and higher level analysis.
Regarding data handling, storage and analysis of gene expression data we have been cooperating with the Linnaeus Centre for Bioinformatics and we are using the LIMS BASE and the connected analysis environment LCB Data Warehouse, but we also develop new data analysis methods ourselves in our computational medicine group.

Practical information
For information about the services we offer and our fees, please see our website.

Details/theories of technology