Karolinska Center for Transgene Technologies (KCTT)

Facility/Node  
Karolinska Center for Transgene Technologies (KCTT)

Contact person
 Johannes Wilbertz (johannes.wilbertz@cmb.ki.se)

Facility website: http://ki.se/ki/jsp/polopoly.jsp?d=3831&a=1340&l=en

Summary of service
Description: KCTT is a non-commercial, non-profit academic core facility and is located at the Karolinska Campus-North (CMB) and South (Novum). The aim of KCTT is to produce transgenic mice by the pronuclear injection and ES cell techniques for research groups at the Karolinska Institute and research groups at other academic institutions. Companies interested in KCTTs service should contact Karolinska Research Services AB (KARSAB) for further information (Folke.Meijer@kab.ki.se).

Technologies/Methods
Pronucleus Injection (Campus C)
Blastocyst Injection (C, Huddinge H)
Electroporation (C, H)
Re-Derivation (C)
KCTT is one of the largest transgenic core facilities in Scandinavia with a yearly number of 60 - 70 constructs for pronucleus injection, around 30 - 40 electroporations of knockout constructs and 40 - 50 injected ko constructs of targeted ES cells

Practical information
For more information on service and prices please see our website.

Details/theories of technology