SNP KTH

Facility/Node
DNA Typing Center at KTH

Contact person
Jacob Odeberg [jacob@biotech.kth.se]

Facility website: http://www.biotech.kth.se/genetech/cores/SNP_core.html

Summary of service
The facility functions as a national resource centre for large scale analysis of gene variability (single nucleotide polymorphisms, SNPs) based on the PyrosequencingTM technology. It provides the necessary infrastructure and competence to function as a central data producing and analysis unit for large scale genotyping projects, and to enable large scale SNP discovery projects through resequencing of candidate genes, taking advantage of infrastructure in the KTH high throughput DNA sequencing facility.
The facility is accredited by the Swedish accreditation agency SWEDAC.

Technologies/Methods
The SNP genotyping facility is built around the Pyrosequencing technology and integrated with purpose designed robotic workstations for stream-lined sample handling and preparation. Two production lines are available.
• A semi-automated line based on the 96 well format PSQ96 Pyrosequencer with in-house designed PSS robots from Magnetic Biosolutions.
• A high throughput line based on a modular MBS (Magnetic Biosolutions) robote infrastructure and 96 HS 2nd generation Pyrosequencer.

Practical information
External projects are run on a research collaboration basis, and the resource centre provides competence and expert advice to external research collaborators prior to setting up and executing SNP genotyping and SNP discovery projects.

Details/theories of technology